Journal: Marine Drugs

Article Title: Fucoxanthin Enhances Cisplatin-Induced Cytotoxicity via NFκB-Mediated Pathway and Downregulates DNA Repair Gene Expression in Human Hepatoma HepG2 Cells

doi: 10.3390/md11010050

Figure Lengend Snippet: ( A ) The ratio of Bax/Bcl-2 mRNA in HepG2 cells pretreated with fucoxanthin (1–10 μM) for 24 h followed by incubation with cisplatin (10 μM) for 24 h. ( B ) The ratio of Bax/Bcl-2 mRNA in HepG2 cells pretreated with fucoxanthin (5 μM) for 24 h followed by incubation with NFκB activation inhibitor (NAI, 20 μΜ) for 2 h and then treated cisplatin (10 μM) for 24 h. Values are means ± SD, n = 3; means without a common letter differ significantly, P < 0.05.

Article Snippet: NFκB activation inhibitor was purchased from Merck Millipore (Billerica, MA, USA).

Techniques: Incubation, Activation Assay

Journal: Molecular Therapy

Article Title: Local Delivery of miR-21 Stabilizes Fibrous Caps in Vulnerable Atherosclerotic Lesions

doi: 10.1016/j.ymthe.2018.01.011

Figure Lengend Snippet: miR-21 Deficiency Activates NF-kB Nuclear Translocation and Enhances OxLDL Uptake in Mouse Peritoneal Macrophages

Article Snippet: To investigate the mechanism, 9 μM NF-kB nucleus translocation inhibitor SN50 (Merck) was added to cultured macrophages before oxLDL loading.

Techniques: Translocation Assay

Journal: Cancers

Article Title: Tumour Necrosis Factor-Alpha (TNF-α)-Induced Metastatic Phenotype in Colorectal Cancer Epithelial Cells: Mechanistic Support for the Role of MicroRNA-21

doi: 10.3390/cancers15030627

Figure Lengend Snippet: NF-kB involvement in TNF-α-induced VEGF in SW480. Cells were treated with 0.13 μM of Bortezomib (BZ) with TNF-α dose range of (0.01–100 pg/mL) for 24 h. Significant differences are shown for comparisons between inhibitor treated cells vs. untreated cells (+ p < 0.05, ++ p < 0.01, ++++ p < 0.0001) and compared with vehicle control (no TNF-α) (* p < 0.05, ** p < 0.01, **** p < 0.0001). Significance was assessed using one-way ANOVA (GraphPad Prism 8). Data are presented as a mean and error bars represent the SEM for six replicates and two independent cultures.

Article Snippet: In some experiments, an NF-kB inhibitor, bortezomib (BZ, 0.13 mM) (LC laboratories), was included in incubations 4.5 h prior to the TNF-α treatments, as indicated in the text and figure legends [ ].

Techniques: Control